Phenotypic and genotypic detection of biofilm formation and methicillin resistance among Staphylococcus aureus isolates

Back ground: S. aureus especially methicillin and multi drug resistant (MRSA MDRSA) has the tendency to form biofilm. Our aim was study in vitro biofilm-forming ability of isolates examine relationship between biofilm formation antibiotic resistance pattern. Methods: One hundred forty one clinical were isolated from wound pus tracheal aspirate samples. Biofilm these detected by Congo red (CRA) method micro-titer plate (MTP) methods confirmed PCR through detection genes. Results: The percentage biofilm-producing found be 81.6% 58.2% for MTP CRA respectively. majority MRSAisolates positive Conclusions: Biofilm-producing exhibit high develop multidrug resistance.